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ti e inverted fluorescence wide field microscope  (Nikon)


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    Structured Review

    Nikon ti e inverted fluorescence wide field microscope
    Ti E Inverted Fluorescence Wide Field Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59743 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+inverted+wide+field+fluorescence+microscope/Objectives/10__1038_slash_s42005___025___02392___8-265-9-8
    Average 99 stars, based on 59743 article reviews
    ti e inverted fluorescence wide field microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
    Article Snippet: After 48 hours, cells were fixed with 4% paraformaldehyde (diluted from 16% paraformaldehyde aqueous solution, Electron Microscopy Sciences) for 15 min and washed with PBS for three times. .. Then cells were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 10x air objective (Nikon PlanFluor 0.3 NA), a motorized stage (ASI), and an sCMOS camera (Hamamatsu ORCA Flash 4.0). ..

    Article Title: Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis
    Article Snippet: Cover slips were then mounted on glass slides using ProLong Diamond Antifade mountant (Thermo Fisher Scientific). .. Samples were cured over night at room temperature and then imaged using a Nikon Ti-E inverted wide-field fluorescence microscope as above, but at 60x magnification (oil immersion, NA 1.4). .. GFP-spot intensity was measured with a macro in ImageJ.

    Article Title: Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis
    Article Snippet: .. Images were acquired using a Nikon Ti-E inverted wide-field fluorescence microscope, paired with a Lumencor SpectraX illumination source. .. To observe live samples, cells were incubated in an Okolab Uno-combined controller stage top incubation chamber to ensure consistent heat, humidity, and 5% CO 2 .

    Article Title: Improved split fluorescent proteins for endogenous protein labeling
    Article Snippet: Most of live-cell imaging was acquired on an inverted Nikon Ti-E microscope (UCSF Nikon Imaging Center), a Yokogawa CSU-W1 confocal scanner unit, a PlanApo VC 100x/1.4NA oil immersion objective, a stage incubator, an Andor Zyla 4.2 sCMOS or an Andor iXon Ultra DU888 EMCCD camera and MicroManager software. .. PAsfCherry2 photoactivation in H2B labeling and split mNG2 vs. split GFP comparison in H2B labeling were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 100X NA 1.40 PlanApo oil immersion objective, a motorized stage (ASI) and an sCMOS camera (Hamamatsu Flash 4.0). .. Microscopy images were subjected to background subtraction using a rolling ball radius of 100 pixels in ImageJ Fiji software.

    Article Title: Bright split red fluorescent proteins with enhanced complementation efficiency for the tagging of endogenous proteins and visualization of synapses
    Article Snippet: .. 48 hours after transfection, cells were fixed with 4% paraformaldehyde and then imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 40× 0.55 NA air objective (Nikon), a motorized stage (ASI) and an sCMOS camera (Tucsen). .. Live-cell imaging ( ) of sorted successful knock-in cells (with either sfCherry3C 1-10 or sfCherry3V 1-10 infection) was acquired on an inverted Nikon Ti-E microscope (UCSF Nikon Imaging Center), a Yokogawa CSU-W1 confocal scanner unit, a Plan Apo VC 100× 1.4 NA oil immersion objective, a stage incubator, an Andor Zyla 4.2 sCMOS camera and MicroManager2.0 software.

    Article Title: Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
    Article Snippet: After 48 hours, cells were fixed with 4% paraformaldehyde (diluted from 16% paraformaldehyde aqueous solution, Electron Microscopy Sciences) for 15 min and washed with PBS for three times. .. Then cells were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 10x air objective (Nikon PlanFluor 0.3 NA), a motorized stage (ASI), and an sCMOS camera (Hamamatsu ORCA Flash 4.0). ..

    Article Title: Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a ×100 NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and a motorized stage (ASI) with stage incubator (Tokai Hit). ..

    Article Title: CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a 100x NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and an motorized stage (ASI) with stage incubator (Tokai Hit). ..

    Microscopy:

    Article Title: Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
    Article Snippet: After 48 hours, cells were fixed with 4% paraformaldehyde (diluted from 16% paraformaldehyde aqueous solution, Electron Microscopy Sciences) for 15 min and washed with PBS for three times. .. Then cells were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 10x air objective (Nikon PlanFluor 0.3 NA), a motorized stage (ASI), and an sCMOS camera (Hamamatsu ORCA Flash 4.0). ..

    Article Title: Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis
    Article Snippet: Cover slips were then mounted on glass slides using ProLong Diamond Antifade mountant (Thermo Fisher Scientific). .. Samples were cured over night at room temperature and then imaged using a Nikon Ti-E inverted wide-field fluorescence microscope as above, but at 60x magnification (oil immersion, NA 1.4). .. GFP-spot intensity was measured with a macro in ImageJ.

    Article Title: Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis
    Article Snippet: .. Images were acquired using a Nikon Ti-E inverted wide-field fluorescence microscope, paired with a Lumencor SpectraX illumination source. .. To observe live samples, cells were incubated in an Okolab Uno-combined controller stage top incubation chamber to ensure consistent heat, humidity, and 5% CO 2 .

    Article Title: Improved split fluorescent proteins for endogenous protein labeling
    Article Snippet: Most of live-cell imaging was acquired on an inverted Nikon Ti-E microscope (UCSF Nikon Imaging Center), a Yokogawa CSU-W1 confocal scanner unit, a PlanApo VC 100x/1.4NA oil immersion objective, a stage incubator, an Andor Zyla 4.2 sCMOS or an Andor iXon Ultra DU888 EMCCD camera and MicroManager software. .. PAsfCherry2 photoactivation in H2B labeling and split mNG2 vs. split GFP comparison in H2B labeling were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 100X NA 1.40 PlanApo oil immersion objective, a motorized stage (ASI) and an sCMOS camera (Hamamatsu Flash 4.0). .. Microscopy images were subjected to background subtraction using a rolling ball radius of 100 pixels in ImageJ Fiji software.

    Article Title: Bright split red fluorescent proteins with enhanced complementation efficiency for the tagging of endogenous proteins and visualization of synapses
    Article Snippet: .. 48 hours after transfection, cells were fixed with 4% paraformaldehyde and then imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 40× 0.55 NA air objective (Nikon), a motorized stage (ASI) and an sCMOS camera (Tucsen). .. Live-cell imaging ( ) of sorted successful knock-in cells (with either sfCherry3C 1-10 or sfCherry3V 1-10 infection) was acquired on an inverted Nikon Ti-E microscope (UCSF Nikon Imaging Center), a Yokogawa CSU-W1 confocal scanner unit, a Plan Apo VC 100× 1.4 NA oil immersion objective, a stage incubator, an Andor Zyla 4.2 sCMOS camera and MicroManager2.0 software.

    Article Title: Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
    Article Snippet: After 48 hours, cells were fixed with 4% paraformaldehyde (diluted from 16% paraformaldehyde aqueous solution, Electron Microscopy Sciences) for 15 min and washed with PBS for three times. .. Then cells were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 10x air objective (Nikon PlanFluor 0.3 NA), a motorized stage (ASI), and an sCMOS camera (Hamamatsu ORCA Flash 4.0). ..

    Article Title: Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a ×100 NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and a motorized stage (ASI) with stage incubator (Tokai Hit). ..

    Article Title: CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a 100x NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and an motorized stage (ASI) with stage incubator (Tokai Hit). ..

    Labeling:

    Article Title: Improved split fluorescent proteins for endogenous protein labeling
    Article Snippet: Most of live-cell imaging was acquired on an inverted Nikon Ti-E microscope (UCSF Nikon Imaging Center), a Yokogawa CSU-W1 confocal scanner unit, a PlanApo VC 100x/1.4NA oil immersion objective, a stage incubator, an Andor Zyla 4.2 sCMOS or an Andor iXon Ultra DU888 EMCCD camera and MicroManager software. .. PAsfCherry2 photoactivation in H2B labeling and split mNG2 vs. split GFP comparison in H2B labeling were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 100X NA 1.40 PlanApo oil immersion objective, a motorized stage (ASI) and an sCMOS camera (Hamamatsu Flash 4.0). .. Microscopy images were subjected to background subtraction using a rolling ball radius of 100 pixels in ImageJ Fiji software.

    Comparison:

    Article Title: Improved split fluorescent proteins for endogenous protein labeling
    Article Snippet: Most of live-cell imaging was acquired on an inverted Nikon Ti-E microscope (UCSF Nikon Imaging Center), a Yokogawa CSU-W1 confocal scanner unit, a PlanApo VC 100x/1.4NA oil immersion objective, a stage incubator, an Andor Zyla 4.2 sCMOS or an Andor iXon Ultra DU888 EMCCD camera and MicroManager software. .. PAsfCherry2 photoactivation in H2B labeling and split mNG2 vs. split GFP comparison in H2B labeling were imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 100X NA 1.40 PlanApo oil immersion objective, a motorized stage (ASI) and an sCMOS camera (Hamamatsu Flash 4.0). .. Microscopy images were subjected to background subtraction using a rolling ball radius of 100 pixels in ImageJ Fiji software.

    Transfection:

    Article Title: Bright split red fluorescent proteins with enhanced complementation efficiency for the tagging of endogenous proteins and visualization of synapses
    Article Snippet: .. 48 hours after transfection, cells were fixed with 4% paraformaldehyde and then imaged on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with an LED light source (Excelitas X-Cite XLED1), a 40× 0.55 NA air objective (Nikon), a motorized stage (ASI) and an sCMOS camera (Tucsen). .. Live-cell imaging ( ) of sorted successful knock-in cells (with either sfCherry3C 1-10 or sfCherry3V 1-10 infection) was acquired on an inverted Nikon Ti-E microscope (UCSF Nikon Imaging Center), a Yokogawa CSU-W1 confocal scanner unit, a Plan Apo VC 100× 1.4 NA oil immersion objective, a stage incubator, an Andor Zyla 4.2 sCMOS camera and MicroManager2.0 software.

    CRISPR:

    Article Title: Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a ×100 NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and a motorized stage (ASI) with stage incubator (Tokai Hit). ..

    Article Title: CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a 100x NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and an motorized stage (ASI) with stage incubator (Tokai Hit). ..

    Imaging:

    Article Title: Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a ×100 NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and a motorized stage (ASI) with stage incubator (Tokai Hit). ..

    Article Title: CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
    Article Snippet: .. CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a 100x NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and an motorized stage (ASI) with stage incubator (Tokai Hit). ..



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